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Image Search Results
Journal: JCI Insight
Article Title: Schwannoma development is mediated by Hippo pathway dysregulation and modified by RAS/MAPK signaling
doi: 10.1172/jci.insight.141514
Figure Lengend Snippet: ( A ) Dissection and histological characterization of mouse schwannoma: H&E and IHC of Schwann cell markers (S100β and GFAP), a neural crest marker (Sox10), and collagen IV. ( B ) H&E and IHC of S100β, GFAP, SOX10, and Collagen IV on human schwannoma tissue sections. ( C ) IHC of phospho-Histone H3 on mice and human schwannoma tissue sections. ( D ) Mouse schwannomas were harvested, and tumorsphere cell culture was performed. Tumorspheres were then seeded to fibronectin-coated plates for monolayer culture. Both cytoplasmic and nuclear fractions isolated from these monolayer cultured cells were analyzed by Western blotting. The tumor cells were injected into nude mice s.c. ( n = 6). ( E ) IHC of YAP and TAZ on mouse and human schwannoma tissue sections. Scale bars: 50 μm.
Article Snippet: The primary antibodies used in this study were as follows: rabbit anti–S100 β (Agilent, catalog Z0311, RRID:AB_10013383), rabbit anti-GFAP (Agilent, catalog Z0334, RRID:AB_10013382),
Techniques: Dissection, Marker, Cell Culture, Isolation, Western Blot, Injection